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Chemical analysis and biological testing of a polar fraction of ambient air, diesel engine, and gasoline engine particulate extracts.

机译:对环境空气,柴油发动机和汽油发动机颗粒物提取物的极性成分进行化学分析和生物学测试。

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摘要

Extracts of gasoline and diesel vehicle exhaust and ambient air particles were fractionated into five fractions according to polarity on a silica gel column. Two medium polar fractions showing high genotoxic activity in the Ames test were further subfractionated, using normal-phase high-performance liquid chromatography. Chemical analyses were performed by means of gas chromatography combined with mass spectrometry and flame ionization and detection. The crude extracts, fractions, and subfractions were assayed with the Ames test, with and without S9, and the most abundant compounds in the subfractions are reported.
机译:根据硅胶柱上的极性,将汽油和柴油车辆废气和环境空气颗粒的提取物分为五个馏分。使用正相高效液相色谱法进一步细分两个在Ames试验中显示出高遗传毒性活性的中等极性馏分。化学分析是通过气相色谱与质谱,火焰电离和检测相结合进行的。在有和没有S9的情况下,使用Ames测试分析粗提物,馏分和亚馏分,并报告了亚馏分中最丰富的化合物。

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